Advances in application of genome editing in tomato and recent development of genome editing technology

Recent Advances in Genome Editing Using CRISPR/Cas9

The CRISPR (clustered regularly interspaced short palindromic repeat)-Cas9 (CRISPR-associated nuclease 9) system is a versatile tool for genome engineering that uses a guide RNA (gRNA) to target Cas9 to a specific sequence. This simple RNA-guided genome-editing technology has become a revolutionary tool in biology and has many innovative applications in different fields. In this review, we brie...

Progress in Genome Editing Technology and Its Application in Plants

Genome editing technology (GET) is a versatile approach that has progressed rapidly as a mechanism to alter the genotype and phenotype of organisms. However, conventional genome modification using GET cannot satisfy current demand for high-efficiency and site-directed mutagenesis, retrofitting of artificial nucleases has developed into a new avenue within this field. Based on mechanisms to reco...

Advances in Nucleases Used for Genome Editing

Genome editing is an exciting technology that allows for specific manipulation of complex genomes. While the original tools for genome manipulation had low efficiencies, genome editing tools discovered in the past fifteen years have been widely studied and great efforts have been required to improve their efficiency. This article summarizes how Zinc Finger Nuclease (ZFN), Transcription Activato...

Advances in genome editing technology and its promising application in evolutionary and ecological studies

Genetic modification has long provided an approach for "reverse genetics", analyzing gene function and linking DNA sequence to phenotype. However, traditional genome editing technologies have not kept pace with the soaring progress of the genome sequencing era, as a result of their inefficiency, time-consuming and labor-intensive methods. Recently, invented genome modification technologies, suc...

Genome Editing using NucleofectorTM Technology